ABSTRACT
To determine the role of pS 2 and ITF(both are members of trefoil peptides) in the early phase of epithelial repair of stress induced gastric lesion. Wistar rats were exposed to water immersion restraint stress(WRS) for 4h, gastric mucosal blood flow(GMBF) was measured with LDF 3 flowmeter, and the extent of gastric mucosal lesions was evaluated grossly and histologically. Expression of pS 2 and ITF mRNA was determined with RT PCR, and further examined with immunohistochemistry. The results showed that a single infliction of WRS produced numerous gastric mucosal erosions, but the number of these lesions gradually decreased and GMBF recovered at 2, 4, 8h after stress. The area of gastric mucosal lesion was reduced by 64 9% and GMBF being increased by 89 8% at 8h. Healing of stress induced ulcerations was accompanied by an increase in the expression of pS 2 (0 51?0 14 vs 0 77?0 11, P
ABSTRACT
To explore the relation between the changes in nitric oxide synthase in gastric mucosa induced by physical stress and psychological stress and acute gastric mucosal lesion,Animal model was reproduced with impulsive stimulator of high voltage and stable currency.Ninety-six male SD rats were randomly divided into three groups:control, regular and irregular group.NOS activity was measured by spectrophotometry,and the degree of injury of gastric mucosa was measured by method of Nils Lambecht.Immunohistochemistry and polyantibodies of NOSⅠ,NOSⅡ,NOSⅢ were employed to investigate locations of NOS.The result showed that NOS activity was stable in normal rats but increased significantly in group of psychological stress( P
ABSTRACT
To determine the role of mucosal gene expression for PS 2 (a member of trefoil peptides), transforming growth factor alpha(TGF ?) and COX 2 during gastric adaptation to WRS in rats, Wistar rats were exposed to single or repeated WRS for 4h every other day for up to 6 days. Gastric mucosal blood flow(GMBF) was measured by LDF 3 Flowmeter,the extent of gastric mucosal lesions was evaluated grossly and histologically, and the expression of PS 2, TGF ?,COX 2 mRNA determined by RT PCR,was used to further demonstrate pS 2 and TGF ?,their expression was detected by immunohistochemistry.The results showed that WRS applied once resulted in numerous gastric mucosal erosions,but repeated application WRS ,could induce adaptation to it. The area of gastric mucosal lesions was reduced by 21 99% after four consecutive WRS. This adaptation to WRS was accompanied by increased GMBF and active cell proliferation in the neck region of gastric glands and by increased expression of PS 2 (0 77?0 01 vs 0 37?0 02, P
ABSTRACT
SD rats were exposed to single or repeated water immersion restraint stress (WRS) for 4 h every other day for up to 6 days, during which the extent of gastric mucosal lesions was evaluated grossly and histologically. The expressions of EGF and EGFR in gastric mucosa were assayed by immunohistochemistry. The results showed that single exposure to WRS led to haemorrhagic lesions, but tolerance developed following repeated exposures to WRS. Histological evaluations showed that single exposure to stress conditions led to crater like haemorrhagic necrosis almost extending to the muscularis mucosae. The submucosa was apparently congestive and edematous. Following repeated WRS, however, mucosal haemorrhagic necrosis was significantly attenuated and submucosal edema and congestion apparently lessened. Active regeneration and proliferation of mucosal cells were observed at the same time. The expressions of EGF and EGFR could be detected in normal gastric mucosa. EGF was expressed in cytoplasma mainly in the regenerative zone with weaker expression at the basal portions of the gastric glands. EGFR was mainly distributed on the membrane of cells in the regenerative zone. Single WRS led to significant decreased in the expressions of EGF and EGFR. their expressions were absent in the necrotic region. After repeated WRS, enhanced expression of EGF and its receptors could be observed not only in mucosal cells of regenterative zone but also in other areas including the lumen of gastric glands. The results suggested that single WRS could cause severe gastric mucosal lesions, but gastric mucosa became tolerant to repeated WRS by way of toleration cytoprotection. Gastric adaptation was accompanied by active regeneration of mucosal cells mediated by EGF binding with its receptor, EGFR.
ABSTRACT
The aim of the study was to investigate the effect of Omeprazole on the intragastric pH and the prevention of upper gastrointestinal bleeding in patients undergoing brain surgery. Twenty patients who received brain surgery were randomly divided into two groups. 72 hour intragastric pH monitoring was carried out in all these patients beginning 4 hours before the operation. After the operation, 10 patients in the control group received therapy, the other 10 patients in Omeprazole group received Omeprazole 40 mg intravenously once daily in addition to routine therapy. In brain surgery patients, the intragastric pH increased during the operation ( P
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In 87 ICU patients intramucosal pH (pHi) was measured, and the new simplified acute physiology score (SAPSⅡ) was calculated within the first 24h ICU admission. Eleven(12 6%) patients died in the ICU.The nonsurvivors had a relatively lower pHi(7 03?0 27) and a higher SAPSⅡ(45?11 77),while the svrvivors had a higher pHi (7 42?0 13) and a lower SAPSⅡ(19 58?10 32).The difference was statistically significant( P
ABSTRACT
Objective To determine the changes of caspase 3 and 8 genes and protein expressions during indomethacin (IND) induced gastric mucosal cell apoptosis in vivo . Methods Healthy male SD rats were treated intragastrically with four different doses of IND. The rats were killed 3 hours after IND administration and the TUNEL technique was applied to detect mucosal cell apoptosis. The change of caspase 3 mRNA expression was detected by in situ hybridization and RT PCR techniques and the changes of caspase 3, 8 protein expression were monitored immunohistochemically. Results In the rats of control group, TUNEL assay revealed that only a few apoptotic cells. Oral administration of IND resulted in the appearance of massive TUNEL positive cells. Computer aided image analysis showed that the mean pixels in 30~120mg/kg groups were 6 3 , 8 0 , 12 6 and 17 1 fold that in control group ( P